(Amniotic fluid is the fluid that surrounds the fetus in the uterus.) This reaction can damage the lungs and heart and cause excessive bleeding. We conclude that both TKH-2 immunostaining and serum STN assay are sensitive methods to diagnose patients with AFE. Diagnosis Treatment Amniotic fluid embolism occurs when some amniotic fluid that contains cells or tissue from the fetus enters the woman’s bloodstream and causes a serious reaction in the woman. Seventeen of 19 sera (89%) were diagnosed as AFE by serum TRH-2 level. The diagnosis of AFES is primarily clinical since there are no specific laboratory or radiographic tests that are pathognomonic or specific to the diagnosis of. The serum STN levels (mean ± SD) in patients with AFE (110.8 ± 48.1 U/ml) showed significantly higher concentrations compared with those of patients with non-AFE (17.3 ± 2.6 U/ml) (p <0.01). Remarkable positive TKH-2 stainings were easily seen within the pulmonary vasculature in 14 of the 15 (93%) patients with AFE. The concentration of STN in serum was measured by an immunoradiometric competitive inhibition assay using the monoclonal antibody TKH-2 in a one-step procedure. Tissue sections were stained using the streptavidin-biotin-immunoperoxidase method. The diagnosis usually relies on the presence of one or more of four otherwise unexplained events occurring during parturience 15, 25 Tables Tables1 1 and and3. There is no single pathognomonic clinical or. Nineteen samples of maternal sera with clinical AFE and 120 control sera and 15 specimen of formalin-fixed, paraffin-embedded lung tissue sections were examined in this study. Amniotic fluid embolism essentially remains a diagnosis of exclusion however, a more detailed examination of many AFE cases does show certain common themes. Amniotic fluid embolism (AFE) is a rare occurrence, which accounts for about 10 of all maternal deaths. Amniotic fluid embolism is a rare obstetric emergency, estimated to occur in 2 to 6/100,000 pregnancies. TKH-2 is a sensitive antibody directed to sialyl Tn (STN), NeuAcα2-6GalNAc. Amniotic fluid embolism is a clinical syndrome of hypoxia, hypotension, and coagulopathy that results from entry of fetal antigens into the maternal circulation. The purpose of this study was to evaluate whether serological assays and immunohistochemical staining, employing antibody TKH-2, are sensitive methods for the serological and histologic diagnosis of amniotic fluid embolism (AFE). PDF Download Buy Article Permissions and Reprints Abstract
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